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You should thoroughly understand where the free energy change
comes from which allows production of ATP.
You should understand what individual processes are taking place in
the pathway and how they contribute to the final outcome.
Last (and least) you should have a reasonable familiarity with
names of substrates, products, enzymes etc. This is not an end in
itself but will help you communicate your understanding of the points
above.
Author: Jon Maber
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Introduction to Glycolysis
What do I need to know?
